Researchers modify the microscope to take real-time 3D images of cells


You can learn about cells from 2D pictures. The knowledge about cells is limited, but creating 3D image It is a time-consuming process.Now, scientists have moved from UT Southwest Have developed A new “simple and cost-effective” device that can take photos from multiple angles and can be retrofitted to an existing laboratory microscope. The team stated that their solution – which involves inserting a unit of two rotating mirrors in front of the microscope’s camera – is 100 times faster than converting images from 2D to 3D.

Currently, this process involves collecting hundreds of sample photos, which can be uploaded to graphics software programs as image stacks, and then calculations are performed to provide multiple perspectives. Even with a powerful computer, these two steps can be very time-consuming. However, using their optical equipment, the team found that they could completely bypass this method.

More importantly, they claim that their method is faster because it only requires one camera exposure instead of hundreds of camera frames for the entire 3D image stack. They discovered this technique when they skew the images captured by two ordinary light-sheet microscopes. While experimenting with their optical methods, they realized that when they used an incorrect amount of skew, the projected image seemed to rotate.

“This is aha! Moment,” said Reto Fiolka, an assistant professor in the Ledder Hill Bioinformatics Department at Southwestern University in Texas. “We realized that this could be more than just an optical de-skew method; the system can also be used for other types of microscopes.”

Using their improved microscope, the team imaged the calcium ions carrying signals between nerve cells in the petri dish and observed the circulatory system of zebrafish embryos. They also performed rapid imaging of cancer cells in motion and a beating zebrafish heart. They also apply the optical unit to other microscopes, including light sheets and rotating disc confocal microscopes.

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